A quick search for popular peptide and other compound protocols. See dosing, duration, side effects and other important guidelines before you start.
Semaglutide
Reconstitution
Sema is dosed in small amounts, so a 2 mL fill on a 5 mg vial (2.5 mg/mL) keeps the early micro-doses measurable. Adjust the fill to suit the dose you are running.
Use bacteriostatic water as your diluent.
Add the water slowly, letting it run down the inside wall of the vial rather than firing it onto the powder.
Swirl gently to mix, then leave it to dissolve. Do not shake.
Give it a few minutes. The finished solution should be clear and colourless.
New to mixing peptides? Our general beginner’s guide to reconstitution walks through the basics that apply to any vial.
Suggested Protocol
Weeks 1 to 4: 0.25 mg
Weeks 5 to 8: 0.5 mg
Weeks 9 to 12: 1 mg
Weeks 13 to 16: 1.7 mg
Week 17 onward: 2.4 mg as the target maintenance dose
Hold each step a full 4 weeks
1.7 mg is a recognised fallback maintenance dose
If nausea at 2.4 mg does not settle after a few weeks, dropping back to 1.7 mg is normal and still effective
Dosing Detail
Start at 0.25 mg for 4 weeks. This is a tolerance-building dose and is not expected to do much for weight on its own.
Step up every 4 weeks: 0.25, 0.5, 1.0, 1.7, 2.4 mg, reaching target around week 16.
2.4 mg is the standard maintenance dose for weight, with 1.7 mg as a fallback if 2.4 is not tolerated.
Same day each week. Half-life is about 7 days, so timing is forgiving.
Based on 2 mL of bac water.
Want a different syringe type or more detail? Open the full dose calculator.
Duration
Long-run protocol. Titrate over the first ~16 weeks, then maintain. Stopping commonly leads to weight regain, so plan an exit (taper or maintenance dose) with that in mind.
Notes
Inject subcutaneously, rotating sites.
Nausea is the most reported early effect and a smaller starting dose helps.
Protein and hydration matter as appetite falls.
Storage & Handling
Keep the powdered (lyophilized) vial in a cool, dark place. Refrigerate it for longer-term storage before mixing.
Once reconstituted and properly stored (refrigerated at 2 to 8°C and kept out of direct light), use the vial within 90 days.
Do not freeze a reconstituted vial, and keep it away from heat and sunlight.
If the solution turns cloudy, develops floating particles, or shows any other sign of degradation, stop using that vial.
